Recombinant humaan TC21/RRAS2-eiwit

RAS viral (r-ras) oncogene homolog 2 isoform a, also known as RRAS2, belongs to the Ras like GTPase family that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. It is implicated in the pathogenesis of human cancers. Thus, overexpression of active RRAS2 in EpH4 cells induces tumorigenicity through the phosphoinositide 3-kinase, p38 MAPK, and mTOR pathways, and these cells lose their sensitivity to the normal growth inhibitory role of TGF-beta. Recombinant human RRAS2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein R-Ras2, Related RAS viral (r-ras) oncogene homolog 2, Ras-like protein TC21, Teratocarcinoma oncogene, TC21

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.2 kDa (221aa) confirmed by MALDI-TOF

TC21, R-Ras2, ATGP0396-10 µg, ATGP0396-20 µg, ATGP0396-50 µg, ATGP0396-100 µg, ATGP0396-250 µg, ATGP0396-500 µg, ATGP0396-1 mg, ATGP0396-10, ATGP0396-20, ATGP0396-50, ATGP0396-100, ATGP0396-250, ATGP0396-500, ATGP0396-1

Erdogan M., et al. (2007) J Biol Chem. 282 (38) :27713-20. ; ; Movilla N., et al. (1999) Oncogene. 18 (43) :5860-9. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

Cancer

NP_036382

P62070

Human

MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI RMNVDQAFHE LVRVIRKFQE QECPPSPEPT RKEKDKKGCH C