Recombinant humaan TCEA1-eiwit

TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.5 kDa (325aa) confirmed by MALDI-TOF

TCEA1, Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS, ATGP1534-10 µg, ATGP1534-20 µg, ATGP1534-50 µg, ATGP1534-100 µg, ATGP1534-250 µg, ATGP1534-500 µg, ATGP1534-1 mg, ATGP1534-10, ATGP1534-20, ATGP1534-50, ATGP1534-100, ATGP1534-250, ATGP1534-500, ATGP1534-1

Kulish, D. et al. (2001) Mol. Cell. Biol. 21: 4162-4168.; ; Park H, et al. (1994) Gene 139 (2) : 263-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 1mM DTT, 50 mM NaCl

Epigenomics (Transcription & Translation)

NP_006747

P23193

Human

HMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC