Recombinant humaan TCEA2-eiwit

TCEA2 is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human TCEA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcription elongation factor A protein 2 isoform a, transcription elongation factor A (SII), 2, TFIIS

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36 kDa (322aa) confirmed by MALDI-TOF

TCEA2, Transcription elongation factor A protein 2 isoform a, transcription elongation factor A (SII), 2, TFIIS, ATGP2132-10 µg, ATGP2132-20 µg, ATGP2132-50 µg, ATGP2132-100 µg, ATGP2132-250 µg, ATGP2132-500 µg, ATGP2132-1 mg, ATGP2132-10, ATGP2132-20, ATGP2132-50, ATGP2132-100, ATGP2132-250, ATGP2132-500, ATGP2132-1

Weaver ZA, Kane CM. Mar 1998. Genomics 46 (3) : 516-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.25M NaCl

Epigenomics (Transcription & Translation)

NP_003186

Q15560

Human

MMGKEEE IARIARRLDK MVTKKSAEGA MDLLRELKAM PITLHLLQST RVGMSVNALR KQSSDEEVIA LAKSLIKSWK KLLDASDAKA RERGRGMPLP TSSRDASEAP DPSRKRPELP RAPSTPRITT FPPVPVTCDA VRNKCREMLT AALQTDHDHV AIGADCERLS AQIEECIFRD VGNTDMKYKN RVRSRISNLK DAKNPDLRRN VLCGAITPQQ IAVMTSEEMA SDELKEIRKA MTKEAIREHQ MARTGGTQTD LFTCGKCRKK NCTYTQVQTR SSDEPMTTFV VCNECGNRWK FC