Recombinant humaan TCEB1-eiwit

TCEB1 (elongin C) is a subunit of elongin, which is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template encoded arresting sites. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau (VHL) tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant human TCEB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcription elongation factor B polypeptide 1, SIII, Elongin-C

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.6 kDa (132aa) confirmed by MALDI-TOF

TCEB1, Transcription elongation factor B polypeptide 1, SIII, Elongin-C, ATGP1363-10 µg, ATGP1363-20 µg, ATGP1363-50 µg, ATGP1363-100 µg, ATGP1363-250 µg, ATGP1363-500 µg, ATGP1363-1 mg, ATGP1363-10, ATGP1363-20, ATGP1363-50, ATGP1363-100, ATGP1363-250, ATGP1363-500, ATGP1363-1

Duan DR., et al. (1995) Science. 269 (5229) :1402-6.; ; Aso T., et al. (1995) Science. 269 (5229) :1439-43.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Epigenomics (Transcription & Translation)

NP_005639

Q15369

Human

MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC