Recombinant humaan TDG-eiwit

Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E. coli

Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

48.4 kDa (433aa)

TDG, ATGP1705-10 µg, ATGP1705-20 µg, ATGP1705-50 µg, ATGP1705-100 µg, ATGP1705-250 µg, ATGP1705-500 µg, ATGP1705-1 mg, ATGP1705-10, ATGP1705-20, ATGP1705-50, ATGP1705-100, ATGP1705-250, ATGP1705-500, ATGP1705-1

Um S., et al. (1998) J Biol Chem. 273:20728-20736.; ; Hardeland u., et al. (2002) EMBO J. 21:1456-1464.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 urea, 10% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

AAH37557

Q13569

Human

MEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA