Recombinant humaan TDO2-eiwit

TDO2, also known as TDO, is a ferrous heme enzyme that catalyzes the first and rate-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism. It incorporates oxygen into the indole moiety of tryptophan. It has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin. Recombinant human TDO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tryptophan 23-dioxygenase, Tryptophan 2,3-dioxygenase, TDO, TPH2, TRPO

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50 kDa (426aa) confirmed by MALDI-TOF

TDO2, Tryptophan 23-dioxygenase, Tryptophan 2,3-dioxygenase, TDO, TPH2, TRPO, ATGP0939-10 µg, ATGP0939-20 µg, ATGP0939-50 µg, ATGP0939-100 µg, ATGP0939-250 µg, ATGP0939-500 µg, ATGP0939-1 mg, ATGP0939-10, ATGP0939-20, ATGP0939-50, ATGP0939-100, ATGP0939-250, ATGP0939-500, ATGP0939-1

Murray MF., et al. (2007) Curr Drug Metab. 8 (3) :197-200.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Metabolism

NP_005642

P48775

Human

MSGCPFLGNN FGYTFKKLPV EGSEEDKSQT GVNRASKGGL IYGNYLHLEK VLNAQELQSE TKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG HVRDERNMLK VVSRMHRVSV ILKLLVQQFS ILETMTALDF NDFREYLSPA SGFQSLQFRL LENKIGVLQN MRVPYNRRHY RDNFKGEENE LLLKSEQEKT LLELVEAWLE RTPGLEPHGF NFWGKLEKNI TRGLEEEFIR IQAKEESEEK EEQVAEFQKQ KEVLLSLFDE KRHEHLLSKG ERRLSYRALQ GALMIYFYRE EPRFQVPFQL LTSLMDIDSL MTKWRYNHVC MVHRMLGSKA GTGGSSGYHY LRSTVSDRYK VFVDLFNLST YLIPRHWIPK MNPTIHKFLY TAEYCDSSYF SSDESD