Recombinant humaan TDP-43/TARDBP-eiwit

TARDBP, as known as TAR DNA binding protein, was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It has also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. It has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recombinant human TARDBP protein protein, fused to His-tag at N-terminus, was expressed in E. coli.

TAR DNA binding protein, ALS10, TDP43

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

48.8 kDa (450aa)

TDP43, TARDBP, TAR DNA binding protein, ALS10, TDP43, ATGP2093-10 µg, ATGP2093-20 µg, ATGP2093-50 µg, ATGP2093-100 µg, ATGP2093-250 µg, ATGP2093-500 µg, ATGP2093-1 mg, ATGP2093-10, ATGP2093-20, ATGP2093-50, ATGP2093-100, ATGP2093-250, ATGP2093-500, ATGP2093-1

Ou SH. et al. (1995) J Virol. 69:3584-3596.

Wang W, et al. The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. (Nat Med. 2016) {https://pubmed.ncbi.nlm.nih.gov/27348499/}; ; Nielsen AK, et al. TDP-43-specific Autoantibody Decline in Patients With Amyotrophic Lateral Sclerosis. (Neurol Neuroimmunol Neuroinflamm. 2020) {https://pubmed.ncbi.nlm.nih.gov/33361387/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Epigenomics (Transcription & Translation)

NP_031401.1

Q13148

Human

MSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM