Recombinant humaan TDP1-eiwit

TDP1, also known as tyrosyl-DNA phosphodiesterase 1, is involved in repairing stalled topoisomerase l-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase l and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical meditated DNA double-strand breaks. Recombinant human TDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tyrosyl-DNA phosphodiesterase 1 isoform a, TDP1, Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

69.5 kDa (617aa)

TDP1, ATGP3593-10 µg, ATGP3593-20 µg, ATGP3593-50 µg, ATGP3593-100 µg, ATGP3593-250 µg, ATGP3593-500 µg, ATGP3593-1 mg, ATGP3593-10, ATGP3593-20, ATGP3593-50, ATGP3593-100, ATGP3593-250, ATGP3593-500, ATGP3593-1

Jakobsen AK., et al, (2015) Exp. Mol. Pathol. 99:56-64.; ; Lebedeva NA., et al, (2015) Biosci. Rep. 35:e00230.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_060789.2

Q9NUW8

Human

Ala

MSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP S