Recombinant humaan TDP2-eiwit

TDP2 is a member of a superfamily of divalent cation-dependent phosphodiesterases. The protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. Recombinant human TDP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tyrosyl-DNA phosphodiesterase 2, AD022, dJ30M3.3, EAP2, EAPII, RP1-30M3.3, TTRAP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

43.3 kDa (385aa) confirmed by MALDI-TOF

TDP2, Tyrosyl-DNA phosphodiesterase 2, AD022, dJ30M3.3, EAP2, EAPII, RP1-30M3.3, TTRAP, ATGP2024-10 µg, ATGP2024-20 µg, ATGP2024-50 µg, ATGP2024-100 µg, ATGP2024-250 µg, ATGP2024-500 µg, ATGP2024-1 mg, ATGP2024-10, ATGP2024-20, ATGP2024-50, ATGP2024-100, ATGP2024-250, ATGP2024-500, ATGP2024-1

Vilotti, S., et al. (2012) Cell Death Differ. 19 (3), 488-500; ; Pinel, P., et al. (2012) J. Neurosci. 32 (3), 817-825;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_057698

O95551

Human

MELGSCL EGGREAAEEE GEPEVKKRRL LCVEFASVAS CDAAVAQCFL AENDWEMERA LNSYFEPPVE ESALERRPET ISEPKTYVDL TNEETTDSTT SKISPSEDTQ QENGSMFSLI TWNIDGLDLN NLSERARGVC SYLALYSPDV IFLQEVIPPY YSYLKKRSSN YEIITGHEEG YFTAIMLKKS RVKLKSQEII PFPSTKMMRN LLCVHVNVSG NELCLMTSHL ESTRGHAAER MNQLKMVLKK MQEAPESATV IFAGDTNLRD REVTRCGGLP NNIVDVWEFL GKPKHCQYTW DTQMNSNLGI TAACKLRFDR IFFRAAAEEG HIIPRSLDLL GLEKLDCGRF PSDHWGLLCN LDIIL