Recombinant humaan TFB1M-eiwit

TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. TFB1M stimulates transcription independently of the methyltransferase activity. Recombinant human TFB1M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dimethyladenosine transferase 1, TFB1M (Mitochondrial transcription factor B1)

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.8 kDa (340aa) confirmed by MALDI-TOF

TFB1M, Dimethyladenosine transferase 1, TFB1M (Mitochondrial transcription factor B1), ATGP1165-10 µg, ATGP1165-20 µg, ATGP1165-50 µg, ATGP1165-100 µg, ATGP1165-250 µg, ATGP1165-500 µg, ATGP1165-1 mg, ATGP1165-10, ATGP1165-20, ATGP1165-50, ATGP1165-100, ATGP1165-250, ATGP1165-500, ATGP1165-1

McCulloch V., et al. (2002) Mol. Cell. Biol. 22:1116-1125; ; Gleyzer N., et al. (2005) Mol. Cell. Biol. 25 (4) :1354-66

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_057104

Q8WVM0

Human

QAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNA DVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSV STPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQA FVPKPEVDVG VVHFTPLIQP KIEQPFKLVE KVVQNVFQFR RKYCHRGLRM LFPEAQRLES TGRLLELADI DPTLRPRQLS ISHFKSLCDV YRKMCDEDPQ LFAYNFREEL KRRKSKNEEK EEDDAENYRL