Recombinant humaan TFB2M-eiwit

TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Recombinant human TFB2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dimethyladenosine transferase 2 mitochondrial, Dimethyladenosine transferase 2, mitochondrial, Hkp1, mtTFB2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.8 kDa (401aa) confirmed by MALDI-TOF

TFB2M, Dimethyladenosine transferase 2 mitochondrial, Dimethyladenosine transferase 2, mitochondrial, Hkp1, mtTFB2, ATGP1773-10 µg, ATGP1773-20 µg, ATGP1773-50 µg, ATGP1773-100 µg, ATGP1773-250 µg, ATGP1773-500 µg, ATGP1773-1 mg, ATGP1773-10, ATGP1773-20, ATGP1773-50, ATGP1773-100, ATGP1773-250, ATGP1773-500, ATGP1773-1

Litonin, D., et al. (2010) J. Biol. Chem. 285 (24), 18129-18133; ; Norrbom, J., et al. (2010) Acta Physiol (Oxf) 198 (1), 71-79.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_071761.1

Q9H5Q4

Human

AGRFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN LGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R