Recombinant humaan TFPI-eiwit

TFPI is a protease inhibitor that regulates the tissue factor (TF) -dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The protein inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. Recombinant human TFPI protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tissue factor pathway inhibitor, EPI, LACI, TFI, TFPI1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.3 kDa (299aa)

TFPI, tissue factor pathway inhibitor, EPI, LACI, TFI, TFPI1, ATGP2333-10 µg, ATGP2333-20 µg, ATGP2333-50 µg, ATGP2333-100 µg, ATGP2333-250 µg, ATGP2333-500 µg, ATGP2333-1 mg, ATGP2333-10, ATGP2333-20, ATGP2333-50, ATGP2333-100, ATGP2333-250, ATGP2333-500, ATGP2333-1

Prabhakar, P., et al. (2012) Thromb. Res. 130 (4), 687-689 ; ; Alioglu, B., et al. (2012) J Clin Res Pediatr Endocrinol 4 (3), 146-150;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Cardiovascular

NP_006278

P10646

Human

DSEEDEE HTIITDTELP PLKLMHSFCA FKADDGPCKA IMKRFFFNIF TRQCEEFIYG GCEGNQNRFE SLEECKKMCT RDNANRIIKT TLQQEKPDFC FLEEDPGICR GYITRYFYNN QTKQCERFKY GGCLGNMNNF ETLEECKNIC EDGPNGFQVD NYGTQLNAVN NSLTPQSTKV PSLFEFHGPS WCLTPADRGL CRANENRFYY NSVIGKCRPF KYSGCGGNEN NFTSKQECLR ACKKGFIQRI SKGGLIKTKR KRKKQRVKIA YEEIFVKNM