Recombinant humaan TFPI-eiwit

TFPI, also known as tissue factor pathway inhibitor isoform, is the major regulator of tissue factor (TF) -induced coagulation. It is predominantly produced by microvascular endothelial cells, though it is also found in platelets, monocytes, smooth muscle cells, and plasma. It is an anticoagulant protein that inhibits early phases of the procoagulant response. It has been shown to be associated with breast cancer pathogenesis. It consists of a highly negatively charged amino-terminus, three tandemly linked Kunitz domains, and a highly positively charged carboxy-terminus. It exhibits significant homology with human inter-alpha-trypsin inhibitor and bovine basic pancreatic trypsin inhibitor. Recombinant human TFPI, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tissue factor pathway inhibitor isoform, TFPI, EPI, LACI, TFI, TFPI1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

33 kDa (285aa)

TFPI, Tissue factor pathway inhibitor isoform, TFPI, EPI, LACI, TFI, TFPI1, ATGP3609-10 µg, ATGP3609-20 µg, ATGP3609-50 µg, ATGP3609-100 µg, ATGP3609-250 µg, ATGP3609-500 µg, ATGP3609-1 mg, ATGP3609-10, ATGP3609-20, ATGP3609-50, ATGP3609-100, ATGP3609-250, ATGP3609-500, ATGP3609-1

Maroney SA., et al. (2010) Thromb Res. 1:S52-S56.; ; Ali HO., et al. (2016) PLoS One. 11:e0152114.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_006278.1

P10646

Human

Ala

DSEEDEE HTIITDTELP PLKLMHSFCA FKADDGPCKA IMKRFFFNIF TRQCEEFIYG GCEGNQNRFE SLEECKKMCT RDNANRIIKT TLQQEKPDFC FLEEDPGICR GYITRYFYNN QTKQCERFKY GGCLGNMNNF ETLEECKNIC EDGPNGFQVD NYGTQLNAVN NSLTPQSTKV PSLFEFHGPS WCLTPADRGL CRANENRFYY NSVIGKCRPF KYSGCGGNEN NFTSKQECLR ACKKGFIQRI SKGGLIKTKR KRKKQRVKIA YEEIFVKNM