Recombinant humaan THG1L-eiwit

Probable tRNA (His) guanylyltransferase, also known as THG1L, belongs to the tRNA (His) gyanylytransferase family. Localized to the cytoplasm, THG1L is also found near the nuclear membrane and is expressed in many tissues, including liver and lung. upon DNA damage, THG1L is phosphorylated, most likely by ATM or ATR. It is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. Recombinant human THG1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Probable tRNA (His) guanylyltransferase, ICF45

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34 kDa (292aa) confirmed by MALDI-TOF

THG1L, Probable tRNA (His) guanylyltransferase, ICF45, ATGP1668-10 µg, ATGP1668-20 µg, ATGP1668-50 µg, ATGP1668-100 µg, ATGP1668-250 µg, ATGP1668-500 µg, ATGP1668-1 mg, ATGP1668-10, ATGP1668-20, ATGP1668-50, ATGP1668-100, ATGP1668-250, ATGP1668-500, ATGP1668-1

Guo D., et al. (2004) J Biol Chem. 279 (51) : 53498-505.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Cell Cycle

NP_060342

Q9NWX6

Human

MGSSHHHHHH SSGLVPRGSH MGSMAKSKFE YVRDFEADDT CLAHCWVVVR LDGRNFHRFA EKHNFAKPND SRALQLMTKC AQTVMEELED IVIAYGQSDE YSFVFKRKTN WFKRRASKFM THVASQFASS YVFYWRDYFE DQPLLYPPGF DGRVVVYPSN QTLKDYLSWR QADCHINNLY NTVFWALIQQ SGLTPVQAQG RLQGTLAADK NEILFSEFNI NYNNELPMYR KGTVLIWQKV DEVMTKEIKL PTEMEGKKMA VTRTRTKPVP LHCDIIGDAF WKEHPEILDE DS