Recombinant humaan Thioredoxin-1-eiwit

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human TXN protein, fused to His-tag at N-terminus, was overexpressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Thioredoxin, TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR.

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

13.9 kDa (125aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Thioredoxin1, Thioredoxin, TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR., ATGP0530-10 µg, ATGP0530-20 µg, ATGP0530-50 µg, ATGP0530-100 µg, ATGP0530-250 µg, ATGP0530-500 µg, ATGP0530-1 mg, ATGP0530-10, ATGP0530-20, ATGP0530-50, ATGP0530-100, ATGP0530-250, ATGP0530-500, ATGP0530-1

Pigiet VP., et al. (1986) Proc Natl Acad Sci uSA. 83 (20) :7643-7. ; ; Lundstrom J., et al. (1990) J Biol Chem. 265 (16) :9114-20.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Redox Proteins

NP_003320

P10599

Human

MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV