Recombinant humaan Thioredoxin Reductase 1/TXNRD1-eiwit

TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 15unit/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

55.7 kDa (508aa) confirmed by MALDI-TOF

GRIM 12, GRIM12, GRIM-12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, Thioredoxin reductase GRIM12, TR, TR 1, TR1, TR-1, TRXR 1, TRXR1, TRXR-1, TXNR, TXNRD 1, TXNRD1, TXNRD-1, ATGP0570-10 µg, ATGP0570-20 µg, ATGP0570-50 µg, ATGP0570-100 µg, ATGP0570-250 µg, ATGP0570-500 µg, ATGP0570-1 mg, ATGP0570-010, ATGP0570-020, ATGP0570-050, ATGP0570-100, ATGP0570-250, ATGP0570-500, ATGP0570-01M

Ma X. et al. (2002) J Biol Chem. 277 (25) :22460-8.; ; Javvadi P. (2010) Cancer Res. 70 (5) :1941-50.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Redox Proteins

NP_001087240.1

Q16881

Human

YDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC