Recombinant humaan THOC7-eiwit

THOC7 is required for efficient export of polyadenylated RNA. This protein acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Recombinant human THOC7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

THO complex subunit 7 homolog, fSAP24, hTREX30, NIF3L1BP1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.1 kDa (227aa) confirmed by MALDI-TOF

THOC7, THO complex subunit 7 homolog, fSAP24, hTREX30, NIF3L1BP1, ATGP2943-10 µg, ATGP2943-20 µg, ATGP2943-50 µg, ATGP2943-100 µg, ATGP2943-250 µg, ATGP2943-500 µg, ATGP2943-1 mg, ATGP2943-10, ATGP2943-20, ATGP2943-50, ATGP2943-100, ATGP2943-250, ATGP2943-500, ATGP2943-1

El Bounkari O., et al. (2009) FEBS Lett. 583 (1), 13-18

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 50% glycerol, 1mM DTT

Epigenetics and Nuclear Signaling

NP_079351

Q6I9Y2

Human

MGAVTDD EVIRKRLLID GDGAGDDRRI NLLVKSFIKW CNSGSQEEGY SQYQRMLSTL SQCEFSMGKT LLVYDMNLRE MENYEKIYKE IECSIAGAHE KIAECKKQIL QAKRIRKNRQ EYDALAKVIQ HHPDRHETLK ELEALGKELE HLSHIKESVE DKLELRRKQF HVLLSTIHEL QQTLENDEKL SEVEEAQEAS METDPKP