Recombinant humaan THTPA-eiwit

THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8. 5. Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Thiamine-triphosphatase, MGC2652, THTP, THTPASE, Thiamine-triphosphatase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.7 kDa (250aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

THTPA, Thiamine-triphosphatase, MGC2652, THTP, THTPASE, Thiamine-triphosphatase, ATGP0676-10 µg, ATGP0676-20 µg, ATGP0676-50 µg, ATGP0676-100 µg, ATGP0676-250 µg, ATGP0676-500 µg, ATGP0676-1 mg, ATGP0676-10, ATGP0676-20, ATGP0676-50, ATGP0676-100, ATGP0676-250, ATGP0676-500, ATGP0676-1

Lakaye B., et al. (2002) J Biol Chem. 277 (16) :13771-7. ; ; Lakaye B., et al. (2004) Int J Biochem Cell Biol. 36 (7) :1348-64.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Enzymes & Proteases

NP_001119811

Q9BU02

Human

MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG