Recombinant humaan Thymidine Kinase 1-eiwit

TK1, also known as thymidine kinase, is an enzyme, a phosphotransferase (a kinase) : 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. It is present in two forms in mammalian cells, TK1 and TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Deoxythymidine is present in the body fluids as a result of degradation of DNA from food and from dead cells. Thymidine kinase is required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry it is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease, mainly of hematological malignancies. Recombinant human TK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Thymidine kinase cytosolic, Thymidine kinase, cytosolic, TK2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28 kDa (258aa) confirmed by MALDI-TOF

Thymidine Kinase1, ATGP1610-10 µg, ATGP1610-20 µg, ATGP1610-50 µg, ATGP1610-100 µg, ATGP1610-250 µg, ATGP1610-500 µg, ATGP1610-1 mg, ATGP1610-10, ATGP1610-20, ATGP1610-50, ATGP1610-100, ATGP1610-250, ATGP1610-500, ATGP1610-1

Chang Z.F., et al. (1998) J. Biol. Chem. 273:12095-12100; ; Kreidberg J.A., et al. (1986) Mol. Cell. Biol. 6:2903-2909

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_003249.3

P04183

Human

MSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN