Recombinant humaan Thymidylaatsynthase/TYMS-eiwit

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu) . Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.8 kDa (333aa) confirmed by MALDI-TOF

Thymidylate synthase, HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase, ATGP0446-10 µg, ATGP0446-20 µg, ATGP0446-50 µg, ATGP0446-100 µg, ATGP0446-250 µg, ATGP0446-500 µg, ATGP0446-1 mg, ATGP0446-10, ATGP0446-20, ATGP0446-50, ATGP0446-100, ATGP0446-250, ATGP0446-500, ATGP0446-1

Chiu TH., et al. (2009) Anticancer Res. 29 (11) :4503-11. ; ; Pena MM., et al. (2009) J Biol Chem. 284 (46) :31597-607.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Enzymes & Proteases

NP_001062

P04818

Human

MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV