Recombinant humaan TIAL1-eiwit

TIAL1 is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. Recombinant human TIAL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44 kDa (398aa) confirmed by MALDI-TOF

TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, ATGP2906-10 µg, ATGP2906-20 µg, ATGP2906-50 µg, ATGP2906-100 µg, ATGP2906-250 µg, ATGP2906-500 µg, ATGP2906-1 mg, ATGP2906-10, ATGP2906-20, ATGP2906-50, ATGP2906-100, ATGP2906-250, ATGP2906-500, ATGP2906-1

Kim HS., et al. (2013) RNA Biol 10 (4), 579-589; ; Singh NN., et al. (2011) Mol. Cell. Biol. 31 (5), 935-954;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 50% glycerol, 2mM DTT, 1mM EDTA

Epigenomics (Transcription & Translation)

NP_003243

Q01085

Human

MGSSHHHHHH SSGLVPRGSH MGSMMEDDGQ PRTLYVGNLS RDVTEVLILQ LFSQIGPCKS CKMITEHTSN DPYCFVEFYE HRDAAAALAA MNGRKILGKE VKVNWATTPS SQKKDTSNHF HVFVGDLSPE ITTEDIKSAF APFGKISDAR VVKDMATGKS KGYGFVSFYN KLDAENAIVH MGGQWLGGRQ IRTNWATRKP PAPKSTQENN TKQLRFEDVV NQSSPKNCTV YCGGIASGLT DQLMRQTFSP FGQIMEIRVF PEKGYSFVRF STHESAAHAI VSVNGTTIEG HVVKCYWGKE SPDMTKNFQQ VDYSQWGQWS QVYGNPQQYG QYMANGWQVP PYGVYGQPWN QQGFGVDQSP SAAWMGGFGA QPPQGQAPPP VIPPPNQAGY GMASYQTQ