Recombinant humaan TIGAR/C12orf5 eiwit

TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TP53-induced glycolysis and apoptosis regulator

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.6 kDa (294aa) confirmed by MALDI-TOF

TIGAR, C12orf5, TP53-induced glycolysis and apoptosis regulator, ATGP0835-10 µg, ATGP0835-20 µg, ATGP0835-50 µg, ATGP0835-100 µg, ATGP0835-250 µg, ATGP0835-500 µg, ATGP0835-1 mg, ATGP0835-10, ATGP0835-20, ATGP0835-50, ATGP0835-100, ATGP0835-250, ATGP0835-500, ATGP0835-1

Bensaad K., et al. (2009) EMBO J. 28 (19) :3015-26.; ; Hasegawa H., et al. (2009) Leukemia. 23 (11) :2090-101.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol

Apoptosis

NP_065108.1

Q9NQ88

Human

MARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR