Recombinant humaan TIGIT-eiwit

TIGIT, also known as T-cell immunoreceptor with Ig and ITIM domains, is a surface protein containing and immunoglobulin variable domain. This protein is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells. Also, it binds with high affinity to the poliovirus receptor which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Recombinant human TIGIT, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

T-cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

40kDa (359aa)

TIGIT, T-cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM, ATGP3704-10 µg, ATGP3704-20 µg, ATGP3704-50 µg, ATGP3704-100 µg, ATGP3704-250 µg, ATGP3704-500 µg, ATGP3704-1 mg, ATGP3704-10, ATGP3704-20, ATGP3704-50, ATGP3704-100, ATGP3704-250, ATGP3704-500, ATGP3704-1

Yu X., et al, (2009) Nat. Immunol. 10:48-57.; ; Godefroy E., et al, (2015) Haematologica 100:1415-1425.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_776160

Q495A1

Human

Met22