Recombinant humaan TIMP-1-eiwit

TIMP1 also known as metalloproteinase inhibitor 1. The matrix metalloproteinases are inhibited by specific endogenous tissue inhibitors of metalloproteinases (TIMPs), which comprise a family of four protease inhibitors: TIMP1, TIMP2, TIMP3 and TIMP4. Overall, all MMPs are inhibited by TIMPs once they are activated but the gelatinases (MMP-2 and MMP-9) can form complexes with TIMPs when the enzymes are in the latent form. The complex of latent MMP-2 (pro-MMP-2) with TIMP-2 serves to facilitate the activation of pro-MMP-2 at the cell surface by MT1-MMP (MMP-14), a membrane-anchored MMP. The role of the pro-MMP-9/TIMP-1 complex is still unknown. Recombinant human TIMP1, fused to His-tag at N-terminus, was expressed in E. coli.

Metalloproteinase inhibitor 1, EPA, EPO, HCI, CLGI, TIMP

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23 kDa (205aa)

TIMP1, Metalloproteinase inhibitor 1, EPA, EPO, HCI, CLGI, TIMP, ATGP3013-10 µg, ATGP3013-20 µg, ATGP3013-50 µg, ATGP3013-100 µg, ATGP3013-250 µg, ATGP3013-500 µg, ATGP3013-1 mg, ATGP3013-10, ATGP3013-20, ATGP3013-50, ATGP3013-100, ATGP3013-250, ATGP3013-500, ATGP3013-1

Brew K., et al. (2000) Biochim Biophys Acta 1477 (1-2) : 267-83.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cardiovascular

NP_003245

P01033

Human

MGSSHHHHHH SSGLVPRGSH MCTCVPPHPQ TAFCNSDLVI RAKFVGTPEV NQTTLYQRYE IKMTKMYKGF QALGDAADIR FVYTPAMESV CGYFHRSHNR SEEFLIAGKL QDGLLHITTC SFVAPWNSLS LAQRRGFTKT YTVGCEECTV FPCLSIPCKL QSGTHCLWTD QLLQGSEKGF QSRHLACLPR EPGLCTWQSL RSQIA