Recombinant humaan TIMP-2-eiwit

TIMP2 is a natural inhibitor of the matrix metalloproteinases, a group of peptidases involved in of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, this protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, TIMP2 may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular. Recombinant human TIMP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TIMP metallopeptidase inhibitor 2, CSC-21K, CSC21K

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.1 kDa (232aa) confirmed by MALDI-TOF

TIMP2, TIMP metallopeptidase inhibitor 2, CSC-21K, CSC21K, ATGP1687-10 µg, ATGP1687-20 µg, ATGP1687-50 µg, ATGP1687-100 µg, ATGP1687-250 µg, ATGP1687-500 µg, ATGP1687-1 mg, ATGP1687-10, ATGP1687-20, ATGP1687-50, ATGP1687-100, ATGP1687-250, ATGP1687-500, ATGP1687-1

Bahudhanapati, H., et al. (2011) J. Biol. Chem. 286 (36), 31761-31770; ; Seo, D.W., et al. (2011) Peptides 32 (9), 1840-1848

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_003246

P16035

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMCS CSPVHPQQAF CNADVVIRAK AVSEKEVDSG NDIYGNPIKR IQYEIKQIKM FKGPEKDIEF IYTAPSSAVC GVSLDVGGKK EYLIAGKAEG DGKMHITLCD FIVPWDTLST TQKKSLNHRY QMGCECKITR CPMIPCYISS PDECLWMDWV TEKNINGHQA KFFACIKRSD GSCAWYRGAA PPKQEFLDIE DP