Recombinant humaan TK2-eiwit

TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK-DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase) : 2-deoxythymidine kinase, ATP-thymidine 5-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2) . A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Thymidine kinase 2 mitochondrial, thymidine kinase 2, mitochondrial, MTDPS2, MTTK

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30.1 kDa (257aa) confirmed by MALDI-TOF

TK2, thymidine kinase 2 mitochondrial, thymidine kinase 2, mitochondrial, MTDPS2, MTTK, ATGP1651-10 µg, ATGP1651-20 µg, ATGP1651-50 µg, ATGP1651-100 µg, ATGP1651-250 µg, ATGP1651-500 µg, ATGP1651-1 mg, ATGP1651-10, ATGP1651-20, ATGP1651-50, ATGP1651-100, ATGP1651-250, ATGP1651-500, ATGP1651-1

Saada A., et al. (2001) Nat. Genet. 29:342-344; ; Kit S. (1985) . Thymidine kinase. Microbiol. Sci. 2 (12) : 369-75.

Aoki H, et al. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA) n. (Cerebellum. 2022) {https://pubmed.ncbi.nlm.nih.gov/35084690/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl, 250 mM Imidazole, 0.1mM PMSF

Enzymes & Proteases

NP_004605.4

O00142

Human

VQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP