Recombinant humaan TL1A/TNFSF15-eiwit

Tumor necrosis factor ligand superfamily member 15, also known TNFSF15, belongs to the TNF superfamily. TNFSF15 is predominantly expressed in endothelial cells and its expression is inducible by TNFalpha and IL1alpha. TNFSF15 is an angiogenesis inhibitor of the TNF family and functions in part by directly inhibiting endothelial cell proliferation. TNFSF15 may act as an autocrine factor to induce apoptosis in endothelial cells via activation of multiple signaling pathways, including stress protein kinases and certain caspases. Recombinant human TNFSF15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Human TL1A/TNFSF15 is coated at 10 µg/mL (100 ul/well) can bind Human DR3/TNFRSF25 (CAT# ATGP4017) in a Functional ELISA assay.

TNF superfamily member 15, TNF ligand-related molecule 1, TL1, vascular endothelial cell growth inhibitor, VEGI, TNF superfamily ligand TL1A, TL1A, vascular endothelial growth inhibitor-192A, VEGI192A

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human DR3/TNFRSF25 (CAT# ATGP4017)

22.7 kDa (201aa) confirmed by MALDI-TOF

TL1A, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TL1, TL1A, VEGI, VEGI192A, ATGP1049-10 µg, ATGP1049-20 µg, ATGP1049-50 µg, ATGP1049-100 µg, ATGP1049-250 µg, ATGP1049-500 µg, ATGP1049-1 mg, ATGP1049-10, ATGP1049-20, ATGP1049-50, ATGP1049-100, ATGP1049-250, ATGP1049-500, ATGP1049-1

Migone T S., et al. (2002) Immunity. 16:479.; ; Zhai Y., et al. (1999) FASEB J.13:181-189.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.15M NaCl, 40% glycerol

Immunology

NP_005109.2

O95150

Human

LKGQEFAPS HQQVYAPLRA DGDKPRAHLT VVRQTPTQHF KNQFPALHWE HELGLAFTKN RMNYTNKFLL IPESGDYFIY SQVTFRGMTS ECSEIRQAGR PNKPDSITVV ITKVTDSYPE PTQLLMGTKS VCEVGSNWFQ PIYLGAMFSL QEGDKLMVNV SDISLVDYTK EDKTFFGAFL L