Recombinant humaan TLR3-eiwit

TLR3, also known as toll-like receptor 3, is a member of the toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. It is innate immune receptors for sensing microbial molecules and damage-associated molecular patterns released from host cells. It recognizes dsRNA and activation of the receptor induces the activation of NF-kappaB and the production of type I interferons (IFNs) . Its signaling activation is associated with ischemic preconditioning-induced protection against brain ischemia and attenuation of reactive astrogliosis. It is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. Recombinant human TLR3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Toll-like receptor 3, TLR3, CD283, IIAE2

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

78.5 kDa (690aa)

TLR3, Toll-like receptor 3, TLR3, CD283, IIAE2, ATGP3761-10 µg, ATGP3761-20 µg, ATGP3761-50 µg, ATGP3761-100 µg, ATGP3761-250 µg, ATGP3761-500 µg, ATGP3761-1 mg, ATGP3761-10, ATGP3761-20, ATGP3761-50, ATGP3761-100, ATGP3761-250, ATGP3761-500, ATGP3761-1

Alexopoulou L., et al. (2001) Nature. 413:732-738.; ; Cole JE., et al. (2011) Proc Natl Acad Sci U S A. 108:2372-2377.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol

Immunology

NP_003256

O15455

Human

Ser23

SSTTKCTVSH EVADCSHLKL TQVPDDLPTN ITVLNLTHNQ LRRLPAANFT RYSQLTSLDV GFNTISKLEP ELCQKLPMLK VLNLQHNELS QLSDKTFAFC TNLTELHLMS NSIQKIKNNP FVKQKNLITL DLSHNGLSST KLGTQVQLEN LQELLLSNNK IQALKSEELD IFANSSLKKL ELSSNQIKEF SPGCFHAIGR LFGLFLNNVQ LGPSLTEKLC LELANTSIRN LSLSNSQLST TSNTTFLGLK WTNLTMLDLS YNNLNVVGND SFAWLPQLEY FFLEYNNIQH LFSHSLHGLF NVRYLNLKRS FTKQSISLAS LPKIDDFSFQ WLKCLEHLNM EDNDIPGIKS NMFTGLINLK YLSLSNSFTS LRTLTNETFV SLAHSPLHIL NLTKNKISKI ESDAFSWLGH LEVLDLGLNE IGQELTGQEW RGLENIFEIY LSYNKYLQLT RNSFALVPSL QRLMLRRVAL KNVDSSPSPF QPLRNLTILD LSNNNIANIN DDMLEGLEKL EILDLQHNNL ARLWKHANPG GPIYFLKGLS HLHILNLESN GFDEIPVEVF KDLFELKIID LGLNNLNTLP ASVFNNQVSL KSLNLQKNLI TSVEKKVFGP AFRNLTELDM RFNPFDCTCE SIAWFVNWIN ETHTNIPELS SHYLCNTPPH YHGFPVRLFD TSSCKDSAPF ELLEHHHHHH