Recombinant humaan TMEFF1/Tomoreguline-1-eiwit

TMEFF1, also known as tomoregulin-1, is a type I transmembrane glycoprotein. This protein contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain of TMEFF1 can be released as a soluble protein. TMEFF1 is predominantly expressed in brain, but is downregulated in brain neoplasms. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto. Recombinant human TMEFF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tomoregulin-1, C9orf2, CT120.1, H7365, TR-1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.9 kDa (314aa) confirmed by MALDI-TOF

TMEFF1, Tomoregulin1, Tomoregulin-1, C9orf2, CT120.1, H7365, TR-1, ATGP2050-10 µg, ATGP2050-20 µg, ATGP2050-50 µg, ATGP2050-100 µg, ATGP2050-250 µg, ATGP2050-500 µg, ATGP2050-1 mg, ATGP2050-10, ATGP2050-20, ATGP2050-50, ATGP2050-100, ATGP2050-250, ATGP2050-500, ATGP2050-1

Gery S, Yin D, et al. (2003) . Oncogene. 22 (18) :2723-7.; ; Harms PW, et al. (2003) . Genes Dev. 17 (21) :2624-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Cancer

NP_003683.2

Q8IYR6

Human

SNQPPGG GGGSGGDCPG GKGKSINCSE LNVRESDVRV CDESSCKYGG VCKEDGDGLK CACQFQCHTN YIPVCGSNGD TYQNECFLRR AACKHQKEIT VIARGPCYSD NGSGSGEGEE EGSGAEVHRK HSKCGPCKYK AECDEDAENV GCVCNIDCSG YSFNPVCASD GSSYNNPCFV REASCIKQEQ IDIRHLGHCT DTDDTSLLGK KDDGLQYRPD VKDASDQRED VYIGNHMPCP ENLNGYCIHG KCEFIYSTQK ASCRCESGYT GQHCEKTDFS ILYVVPSRQK LTHV