Recombinant humaan TMOD3-eiwit

TMOD3 belongs to the tropomodulin family. Regulation of the actin cytoskeleton by filament capping proteins is critical to myriad dynamic cellular functions. The ability of these proteins to bind both filaments as well as monomers is often central to their cellular functions. The ubiquitous pointed end capping protein TMOD3 acts as a negative regulator of cell migration, yet mechanisms behind its cellular functions are not understood. Recombinant human TMOD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tropomodulin-3, Tropomodulin 3, u-Tmod, uTMOD, ubiquitous tropomodulin

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

42 kDa (376aa) confirmed by MALDI-TOF

TMOD3, Tropomodulin-3, Tropomodulin 3, u-Tmod, uTMOD, ubiquitous tropomodulin, ATGP1647-10 µg, ATGP1647-20 µg, ATGP1647-50 µg, ATGP1647-100 µg, ATGP1647-250 µg, ATGP1647-500 µg, ATGP1647-1 mg, ATGP1647-10, ATGP1647-20, ATGP1647-50, ATGP1647-100, ATGP1647-250, ATGP1647-500, ATGP1647-1

Fischer RS., et al. (2006) J Biol Chem. 2006 Nov 24; ;281 (47) :36454-65. Epub 2006 Oct 1.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Cytoskeleton

NP_055362

Q9NYL9

Human

MGSSHHHHHH SSGLVPRGSH MGSHMALPFR KDLEKYKDLD EDELLGNLSE TELKQLETVL DDLDPENALL PAGFRQKNQT SKSTTGPFDR EHLLSYLEKE ALEHKDREDY VPYTGEKKGK IFIPKQKPVQ TFTEEKVSLD PELEEALTSA SDTELCDLAA ILGMHNLITN TKFCNIMGSS NGVDQEHFSN VVKGEKILPV FDEPPNPTNV EESLKRTKEN DAHLVEVNLN NIKNIPIPTL KDFAKALETN THVKCFSLAA TRSNDPVATA FAEMLKVNKT LKSLNVESNF ITGVGILALI DALRDNETLA ELKIDNQRQQ LGTAVELEMA KMLEENTNIL KFGYQFTQQG PRTRAANAIT KNNDLVRKRR VEGDHQ