Recombinant humaan Tmp21/p23 eiwit

TMED10 is a member of the EMP24/GP25L/p24 family and a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. Recombinant human TMED10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transmembrane emp24 domain-containing protein 10, p23, P24 (DELTA), S31I125, S31III125, Tmp-21-I, TMP21

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20 kDa (177aa) confirmed by MALDI-TOF

Tmp21, p23, Transmembrane emp24 domain-containing protein 10, p23, P24 (DELTA), S31I125, S31III125, Tmp-21-I, TMP21, ATGP2253-10 µg, ATGP2253-20 µg, ATGP2253-50 µg, ATGP2253-100 µg, ATGP2253-250 µg, ATGP2253-500 µg, ATGP2253-1 mg, ATGP2253-10, ATGP2253-20, ATGP2253-50, ATGP2253-100, ATGP2253-250, ATGP2253-500, ATGP2253-1

Chen F, Hasegawa H, et al. (2006) . Nature. 440 (7088) :1208-12.; ; Wang H, et al. (2002) . J Biol Chem. 277 (6) :4541-50.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Signal Transduction

NP_006818

P49755

Human

ISFHLPI NSRKCLREEI HKDLLVTGAY EISDQSGGAG GLRSHLKITD SAGHILYSKE DATKGKFAFT TEDYDMFEVC FESKGTGRIP DQLVILDMKH GVEAKNYEEI AKVEKLKPLE VELRRLEDLS ESIVNDFAYM KKREEEMRDT NESTNTR