Recombinant humaan TNFR2/TNFRSF1B eiwit

TNFRSF1B, also known as tumor necrosis factor receptor superfamily member 1B, mediates most of the metabolic effects of TNF-alpha. The receptor isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Further, high plasma levels of TNFRSF1B were significantly associated with increased incidence of coronary heart disease. Recombinant human TNFRSF1B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human TNFRSF1B inhibit cytotoxicity in the L-929 mouse fibroblast cells in the presence of Human TNF-α. The ED50 range ≤ 0.2 μg/ml.

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, Tumor necrosis factor-binding protein 2, TBP-2, TBPII, CD120b, TNFBR, TNFR80, TNF-R75

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its ability to inhibit cytotoxicity using L-929 mouse fibroblast cells in the presence of Human TNF-α. The ED50 range ≤ 0.2 μg/ml.

25.9 kDa (241aa)

TNF RII, TNFRSF1B, TNFRSF1B, CD120b, p75, p75TNFR, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, ATGP3179-10 µg, ATGP3179-20 µg, ATGP3179-50 µg, ATGP3179-100 µg, ATGP3179-250 µg, ATGP3179-500 µg, ATGP3179-1 mg, ATGP3179-10, ATGP3179-20, ATGP3179-50, ATGP3179-100, ATGP3179-250, ATGP3179-500, ATGP3179-1

Maier O et al., (2013) Biochem. Biophys. Res. Commun. 440 (2) :336-341.; ; Govindaraj C et al., (2013) Clin. Immunol. 149 (1) :97-110.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_001057.1

P20333

Human