Recombinant humaan TNNC1-eiwit

Troponin C type 1, also known as TNNC1, is a member of troponins family. Troponin has 3 subunits, Troponin I (Tn-I), Troponin T (Tn-T) and Troponin C (Tn-C) . Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. TNNC1 is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Recombinant human TNNC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Troponin C type 1, CMD1Z, CMH13, TN-C, TNC, TNNC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.5 kDa (181aa) confirmed by MALDI-TOF

TNNC1, Troponin C type 1, CMD1Z, CMH13, TN-C, TNC, TNNC, ATGP1260-10 µg, ATGP1260-20 µg, ATGP1260-50 µg, ATGP1260-100 µg, ATGP1260-250 µg, ATGP1260-500 µg, ATGP1260-1 mg, ATGP1260-10, ATGP1260-20, ATGP1260-50, ATGP1260-100, ATGP1260-250, ATGP1260-500, ATGP1260-1

Wu A H) ., et al. (1998) Clin Chem. 44:1198-1208.; ; Hamm C W., et al. (2001) Thromb Res. 103:S63-S69.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Cytoskeleton

NP_003271

P63316

Human

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E