Recombinant humaan TNRC5/CNPY3-eiwit

Canopy 3 homolog, also known as CNPY3, is Toll-like receptor (TLR) -specific co-chaperone for HSP90B1. This protein is required for proper TLR folding, except that of TLR3, and hence controls TLR exit from the endoplasmic reticulum. Consequently, CNPY3i s required for both innate and adaptive immune responses. Recombinant human CNPY3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Canopy 3 homolog, CAG4A, ERDA5, PRAT4A, TNRC5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.9 kDa (271aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CNPY3, Canopy 3 homolog, CAG4A, ERDA5, PRAT4A, TNRC5, ATGP1994-10 µg, ATGP1994-20 µg, ATGP1994-50 µg, ATGP1994-100 µg, ATGP1994-250 µg, ATGP1994-500 µg, ATGP1994-1 mg, ATGP1994-10, ATGP1994-20, ATGP1994-50, ATGP1994-100, ATGP1994-250, ATGP1994-500, ATGP1994-1

Chen R., et al. (2009) J. Proteome Res. 8:651-661

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Heat Shock Proteins

NP_006577

Q9BT09

Human

GPSQAGA EENDWVRLPS KCEVCKYVAV ELKSAFEETG KTKEVIGTGY GILDQKASGV KYTKSDLRLI EVTETICKRL LDYSLHKERT GSNRFAKGMS ETFETLHNLV HKGVKVVMDI PYELWNETSA EVADLKKQCD VLVEEFEEVI EDWYRNHQEE DLTEFLCANH VLKGKDTSCL AEQWSGKKGD TAALGGKKSK KKSSRAKAAG GRSSSSKQRK ELGGLEGDPS PEEDEGIQKA SPLTHSPPDE L