Recombinant humaan Tollip-eiwit

TOLLIP, also known as Toll interacting protein, is an inhibitory adaptor protein within Toll-like receptors. The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Negative regulation of TLR signaling by TOLLIP may limit the production of proinflammatory mediators during inflammation and infection. Also, it forms a complex with Tom1 to regulate endosomal trafficking of ubiquitinated proteins. Recombinant human TOLLIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Toll interacting protein, FLJ33531, IL-1RAcPIP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.4 kDa (294aa) confirmed by MALDI-TOF

Tollip, Toll interacting protein, FLJ33531, IL-1RAcPIP, ATGP1067-10 µg, ATGP1067-20 µg, ATGP1067-50 µg, ATGP1067-100 µg, ATGP1067-250 µg, ATGP1067-500 µg, ATGP1067-1 mg, ATGP1067-10, ATGP1067-20, ATGP1067-50, ATGP1067-100, ATGP1067-250, ATGP1067-500, ATGP1067-1

Burns K. et al. (2000) Nat. Cell Biol. 2: 346-351.; ; Zhang G. et al. (2002) J. Biol. Chem. 277: 7059-7065.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl,2mM DTT

Immunology

NP_061882.2

Q9H0E2

Human

MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP