Recombinant humaan TOMM20-eiwit

Mmitochondrial import receptor subunit TOMM20 homolog, also known as TOMM20, belongs to the Tom20 family. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. TOMM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Recombinant human TOMM20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Translocase of outer mitochondrial membrane 20, Translocase of outer mitochondrial membrane 20 homolog, Translocase of outer mitochondrial membrane 20 homolog type II, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOM20, MOM19, MAS20

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.2 kDa (144aa) confirmed by MALDI-TOF

TOMM20, ATGP2112-10 µg, ATGP2112-20 µg, ATGP2112-50 µg, ATGP2112-100 µg, ATGP2112-250 µg, ATGP2112-500 µg, ATGP2112-1 mg, ATGP2112-10, ATGP2112-20, ATGP2112-50, ATGP2112-100, ATGP2112-250, ATGP2112-500, ATGP2112-1

Ahting u., et al. (1999) J Cell Biol. 147:959-968.; ; Brix J., et al. (1999) J Biol Chem. 274:16522-16530

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 2mM DTT

Metabolism

NP_055580

Q15388

Human

DRKRRSD PNFKNRLRER RKKQKLAKER AGLSKLPDLK DAEAVQKFFL EEIQLGEELL AQGEYEKGVD HLTNAIAVCG QPQQLLQVLQ QTLPPPVFQM LLTKLPTISQ RIVSAQSLAE DDVE