Recombinant humaan TOMM34 eiwit

TOMM34 is involved in the import of precursor proteins into mitochondria. The protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. Recombinant human TOMM34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitochondrial import receptor subunit TOM34, HTOM34P, TOM34, uRCC3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.9 kDa (332aa) confirmed by MALDI-TOF

TOMM34, mitochondrial import receptor subunit TOM34, HTOM34P, TOM34, uRCC3, ATGP2149-10 µg, ATGP2149-20 µg, ATGP2149-50 µg, ATGP2149-100 µg, ATGP2149-250 µg, ATGP2149-500 µg, ATGP2149-1 mg, ATGP2149-10, ATGP2149-20, ATGP2149-50, ATGP2149-100, ATGP2149-250, ATGP2149-500, ATGP2149-1

Faou, P., et al. (2012) Biochim. Biophys. Acta 1823 (2), 348-357; ; Blesa, J.R., et al. (2008) Biochem. Cell Biol. 86 (1), 46-56

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_006800

Q15785

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPKFPD SVEELRAAGN ESFRNGQYAE ASALYGRALR VLQAQGSSDP EEESVLYSNR AACHLKDGNC RDCIKDCTSA LALVPFSIKP LLRRASAYEA LEKYPMAYVD YKTVLQIDDN VTSAVEGINR MTRALMDSLG PEWRLKLPSI PLVPVSAQKR WNSLPSENHK EMAKSKSKET TATKNRVPSA GDVEKARVLK EEGNELVKKG NHKKAIEKYS ESLLCSNLES ATYSNRALCY LVLKQYTEAV KDCTEALKLD GKNVKAFYRR AQAHKALKDY KSSFADISNL LQIEPRNGPA QKLRQEVKQN LH