Recombinant humaan TPGS2-eiwit

TPGS2 is a protein that is a component of the neuronal polyglutamylase complex, which plays a role in post-translational addition of glutamate residues to C-terminal tubulin tails. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human TPGS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tubulin polyglutamylase complex subunit 2, C18orf10, HMFN0601, HsT3006, L17, PGs2, Polyglutamylase subunit 2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.7 kDa (323aa) confirmed by MALDI-TOF

Tubulin polyglutamylase complex subunit 2, C18orf10, HMFN0601, HsT3006, L17, PGs2, Polyglutamylase subunit 2, TPGS2, ATGP2283-10 µg, ATGP2283-20 µg, ATGP2283-50 µg, ATGP2283-100 µg, ATGP2283-250 µg, ATGP2283-500 µg, ATGP2283-1 mg, ATGP2283-010, ATGP2283-020, ATGP2283-050, ATGP2283-100, ATGP2283-250, ATGP2283-500, ATGP2283-01M

Oh, J.H., et al. (2005) Mamm. Genome 16 (12), 942-954; ; Janke, C., et al. (2002) Science 308 (5729), 1758-1762

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Signal Transduction

NP_056291

Q68CL5

Human

MEEEASS PGLGCSKPHL EKLTLGITRI LESSPGVTEV TIIEKPPAER HMISSWEQKN NCVMPEDVKN FYLMTNGFHM TWSVKLDEHI IPLGSMAINS ISKLTQLTQS SMYSLPNAPT LADLEDDTHE ASDDQPEKPH FDSRSVIFEL DSCNGSGKVC LVYKSGKPAL AEDTEIWFLD RALYWHFLTD TFTAYYRLLI THLGLPQWQY AFTSYGISPQ AKQWFSMYKP ITYNTNLLTE ETDSFVNKLD PSKVFKSKNK IVIPKKKGPV QPAGGQKGPS GPSGPSTSST SKSSSGSGNP TRK