Recombinant humaan TPK1-eiwit

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Thiamin pyrophosphokinase 1, HTPK1, PP20

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.8 kDa (267aa) confirmed by MALDI-TOF

TPK1, Thiamin pyrophosphokinase 1, HTPK1, PP20, ATGP1415-10 µg, ATGP1415-20 µg, ATGP1415-50 µg, ATGP1415-100 µg, ATGP1415-250 µg, ATGP1415-500 µg, ATGP1415-1 mg, ATGP1415-10, ATGP1415-20, ATGP1415-50, ATGP1415-100, ATGP1415-250, ATGP1415-500, ATGP1415-1

Nosaka K., et al. (2001) Biochim. Biophys. Acta. 1517:293-297; ; Zhao R., et al. (2004) Biochim. Biophys. Acta. 1517:320-322

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_071890

Q9H3S4

Human

MEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS