Recombinant humaan TPM1-eiwit

TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. TPM1 is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. Recombinant human TPM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tropomyosin alpha-1 chain, TPM1, CMD1Y, HTM-alpha, TMSA, Tropomyosin-1, Alpha-tropomyosin, Tropomyosin alpha-1 chain

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

35.0 kDa (304aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) )

TPM1, Tropomyosin alpha-1 chain, TPM1, CMD1Y, HTM-alpha, TMSA, Tropomyosin-1, Alpha-tropomyosin, Tropomyosin alpha-1 chain, ATGP0683-10 µg, ATGP0683-20 µg, ATGP0683-50 µg, ATGP0683-100 µg, ATGP0683-250 µg, ATGP0683-500 µg, ATGP0683-1 mg, ATGP0683-10, ATGP0683-20, ATGP0683-50, ATGP0683-100, ATGP0683-250, ATGP0683-500, ATGP0683-1

Lees-Miller JP.. (1991) Bioessays. 13 (9) :429-37.; ; Perry SV. (2001) J Muscle Res Cell Motil. 22 (1) :5-49.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl,1mM EDTA, 0.1mM PMSF

Cytoskeleton

NP_000357.3

P09493

Human

MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGQV RQLEEQLRIM DQTLKALMAA EDKYSQKEDR YEEEIKVLSD KLKEAETRAE FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSM