Recombinant humaan TPM2-eiwit

Tropomyosin beta chain isoform 2, also known as TPM2, is a member of the actin filament binding protein family which mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Recombinant human TPM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tropomyosin beta chain isoform 2, AMCD1, DA1, DA2B, NEM4, TMSB

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

35.1 kDa (304aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

TPM2, Tropomyosin beta chain isoform 2, AMCD1, DA1, DA2B, NEM4, TMSB, ATGP0873-10 µg, ATGP0873-20 µg, ATGP0873-50 µg, ATGP0873-100 µg, ATGP0873-250 µg, ATGP0873-500 µg, ATGP0873-1 mg, ATGP0873-10, ATGP0873-20, ATGP0873-50, ATGP0873-100, ATGP0873-250, ATGP0873-500, ATGP0873-1

Hunt CC., et al. (1995) Cytogenet Cell Genet. 71 (1) :94-5.; ; Tiso N., et al. (1997) Biochem Biophys Res Commun. 13 (2) :347-50.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 100 mM NaCl

Cytoskeleton

NP_998839.1

P07951

Human

MDAIKKKMQM LKLDKENAID RAEQAEADKK QAEDRCKQLE EEQQALQKKL KGTEDEVEKY SESVKEAQEK LEQAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ENRAMKDEEK MELQEMQLKE AKHIAEDSDR KYEEVARKLV ILEGELERSE ERAEVAESRA RQLEEELRTM DQALKSLMAS EEEYSTKEDK YEEEIKLLEE KLKEAETRAE FAERSVAKLE KTIDDLEETL ASAKEENVEI HQTLDQTLLE LNNL