Recombinant humaan TPM3-eiwit

Tropomyosin alpha-3 chain, also known as TPM3, is member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Recombinant human TPM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tropomyosin alpha-3 chain, hscp30, NEM1, OK/SW-cl.5, TM-5, TM3, TM30 TM30 nm, TPMsk, TRK

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.6 kDa (272aa) confirmed by MALDI-TOF

TPM3, Tropomyosin alpha-3 chain, hscp30, NEM1, OK/SW-cl.5, TM-5, TM3, TM30 TM30 nm, TPMsk, TRK, ATGP1368-10 µg, ATGP1368-20 µg, ATGP1368-50 µg, ATGP1368-100 µg, ATGP1368-250 µg, ATGP1368-500 µg, ATGP1368-1 mg, ATGP1368-10, ATGP1368-20, ATGP1368-50, ATGP1368-100, ATGP1368-250, ATGP1368-500, ATGP1368-1

Ohtsuki I., et al. (2002) J Biochem. 131:739-743.; ; Tiso N., et al. (1997) Biochem Biophys Res Commun. 230:347-350.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Cytoskeleton

NP_705935.1

P06753

Human

MAGITT IEAVKRKIQV LQQQADDAEE RAERLQREVE GERRAREQAE AEVASLNRRI QLVEEELDRA QERLATALQK LEEAEKAADE SERGMKVIEN RALKDEEKME LQEIQLKEAK HIAEEADRKY EEVARKLVII EGDLERTEER AELAESRCRE MDEQIRLMDQ NLKCLSAAEE KYSQKEDKYE EEIKILTDKL KEAETRAEFA ERSVAKLEKT IDDLEDKLKC TKEEHLCTQR MLDQTLLDLN EM