Recombinant humaan TPM4-eiwit

TPM4 belongs to the tropomyosin family. Tropomyosins are present in virtually all eukaryotic cells (both muscle and nonmuscle), where they bind actin filaments and function to modulate actin-myosin interaction and stabilize actin filament structure. TPM4 binds to actin filaments in muscle and nonmuscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. Recombinant human TPM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tropomyosin alpha-4 chain, Tropomyosin 4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30.7 kDa (268aa) confirmed by MALDI-TOF

TPM4, Tropomyosin alpha-4 chain, Tropomyosin 4, ATGP0992-10 µg, ATGP0992-20 µg, ATGP0992-50 µg, ATGP0992-100 µg, ATGP0992-250 µg, ATGP0992-500 µg, ATGP0992-1 mg, ATGP0992-10, ATGP0992-20, ATGP0992-50, ATGP0992-100, ATGP0992-250, ATGP0992-500, ATGP0992-1

Lehman W., et al. (2000) J Mol Biol. 302 (3) :593-606. ; ; Wilton SD., et al. (1996) Cytogenet Cell Genet. 72 (4) :294-6.

Karasawa R, et al. Association of Anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis. (Rheumatology (Oxford) . 2023) {https://pubmed.ncbi.nlm.nih.gov/37144941/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Cytoskeleton

NP_003281

P67936

Human

MGSSHHHHHH SSGLVPRGSH MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLNELNCI