Recombinant humaan TPST1-eiwit

TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP) . Recombinant human TPST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Protein-tyrosine sulfotransferase 1, TANGO13A

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

40.6 kDa (354aa)

TPST1, Protein-tyrosine sulfotransferase 1, TANGO13A, ATGP3331-10 µg, ATGP3331-20 µg, ATGP3331-50 µg, ATGP3331-100 µg, ATGP3331-250 µg, ATGP3331-500 µg, ATGP3331-1 mg, ATGP3331-10, ATGP3331-20, ATGP3331-50, ATGP3331-100, ATGP3331-250, ATGP3331-500, ATGP3331-1

Ouyang Y.B., et al. (1998) Proc Natl Acad Sci USA. 95:2896-2901.; ; Danan L.M., et al. (2008) J. Am. Soc. Mass. Spectrom. 19:1459-1466.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_003587

O60507

Human

Ala

QHAMECH HRIEERSQPV KLESTRTTVR TGLDLKANKT FAYHKDMPLI FIGGVPRSGT TLMRAMLDAH PDIRCGEETR VIPRILALKQ MWSRSSKEKI RLDEAGVTDE VLDSAMQAFL LEIIVKHGEP APYLCNKDPF ALKSLTYLSR LFPNAKFLLM VRDGRASVHS MISRKVTIAG FDLNSYRDCL TKWNRAIETM YNQCMEVGYK KCMLVHYEQL VLHPERWMRT LLKFLQIPWN HSVLHHEEMI GKAGGVSLSK VERSTDQVIK PVNVGALSKW VGKIPPDVLQ DMAVIAPMLA KLGYDPYANP PNYGKPDPKI IENTRRVYKG EFQLPDFLKE KPQTEQVE