Recombinant humaan TRACP/PAP/ACS5 eiwit

ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5 was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is >10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C

Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP, HPAP, TRACP5a, TRACP5b, TrATPase

Baculovirus

Human

Non-Tagged

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is >10,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C

34.3 kDa (304aa)

TRACP, PAP, ACP5, Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP, HPAP, TRACP5a, TRACP5b, TrATPase, ATGP3514-10 µg, ATGP3514-20 µg, ATGP3514-50 µg, ATGP3514-100 µg, ATGP3514-250 µg, ATGP3514-500 µg, ATGP3514-1 mg, ATGP3514-10, ATGP3514-20, ATGP3514-50, ATGP3514-100, ATGP3514-250, ATGP3514-500, ATGP3514-1

Janckila A.J. et al., (1992) Clin. Biochem. 25:437-443.; ; Hayman A.R. et al., (1991) Biochem. J. 277:631-634.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001104505

P13686

Human

ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP