Recombinant humaan TRAILR2/TNFRSF10B eiwit

TNFRSF10B, also known as tumor necrosis factor receptor superfamily member 10B isoform 1, is a member of receptors for the cytotoxic ligand TNFSF10/TRAIL. It can be activated by tumor necrosis factor-related apoptosis inducing ligand, and transduces an apoptosis signal. This protein promotes the activation of NF-kappa-B. Defects in this protein causes of head and neck squamous cell carcinomas. Recombinant human TNFRSF10B protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤5 ng/mL with Human TRAIL.

Tumor necrosis factor receptor superfamily member 10B isoform 1, TNFRSF10B, CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TRAIL receptor 2, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤5 ng/mL with Human TRAIL.

43.9 kDa (394aa)

TRAIL R2, TNFRSF10B, ATGP3732-10 µg, ATGP3732-20 µg, ATGP3732-50 µg, ATGP3732-100 µg, ATGP3732-250 µg, ATGP3732-500 µg, ATGP3732-1 mg, ATGP3732-10, ATGP3732-20, ATGP3732-50, ATGP3732-100, ATGP3732-250, ATGP3732-500, ATGP3732-1

Finnberg NK., et al, (2016) Cancer Res. 76:700-712.; ; Shin GC., et al, (2016) Autophagy. 12:2451-2466.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_003833

O14763

Human

Ile56