Recombinant humaan TRAILR4/TNFRSF10D eiwit

TNFRSF10D, also known as tumor necrosis factor receptor superfamily member 10D, is a member of the TNF-receptor superfamily. This protein contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplasmic death domain. Also, it is can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. Recombinant human TNFRSF10D, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤10 ng/mL with Human TRAIL.

Tumor necrosis factor receptor superfamily member 10D, Decoy receptor 2, DcR2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor 4, TRAIL-R4, TRAIL receptor with a truncated death domain, CD264, TRAILR4, TRUNDD

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤10 ng/mL with Human TRAIL.

43.8 kDa (395aa)

TRAIL R4, TNFRSF10D, ATGP3702-10 µg, ATGP3702-20 µg, ATGP3702-50 µg, ATGP3702-100 µg, ATGP3702-250 µg, ATGP3702-500 µg, ATGP3702-1 mg, ATGP3702-10, ATGP3702-20, ATGP3702-50, ATGP3702-100, ATGP3702-250, ATGP3702-500, ATGP3702-1

Degli-Esposti MA., et al, (1997) Immunity 7:813-820.; ; Marsters SA., et al, (1997) Curr. Biol. 7:1003-1006.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_003831

Q9UBN6

Human

Ala56