Recombinant humaan TRAIL/TNFSF10-eiwit

TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL-R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRUNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRUNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL. Recombinant human TRAIL was expressed in E. coli and purified by using conventional chromatography techniques.

Human TRAIL/TNFSF10 stimulates cell cytotoxicity in the Jurkat human acute T cell leukemia cells. The ED50 range ≤4 ng/mL.

Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253, APO2L, TL2, TANCR

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cytotoxicity assay using Jurkat human acute T cell leukemia cells. The ED50 range ≤4 ng/mL.

19.6 kDa (169aa)

TRAIL, TNFSF10, Tumor necrosis factor (ligand) superfamily, member 10, TNFSF10, Apo-2L, APO2L, CD253, TL2, ATGP3588-10 µg, ATGP3588-20 µg, ATGP3588-50 µg, ATGP3588-100 µg, ATGP3588-250 µg, ATGP3588-500 µg, ATGP3588-1 mg, ATGP3588-10, ATGP3588-20, ATGP3588-50, ATGP3588-100, ATGP3588-250, ATGP3588-500, ATGP3588-1

Janssen EM., et al. (2005) . Nature. 434 (7029) :88-93.; ; Deocaris CC., et al. (2007) Ann N Y Acad Sci. 1119:165-75

Park D, et al. Morusin Induces TRAIL Sensitization by Regulating EGFR and DR5 in Human Glioblastoma Cells. (Journal of Natural Products. 2016) {https://pubmed.ncbi.nlm.nih.gov/26829656/}; ; Kim J, et al. Decursin enhances TRAIL-induced apoptosis through oxidative stress mediated- endoplasmic reticulum stress signalling in non-small cell lung cancers. (Br J Pharmacol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26661339/}; ; Kim EO, et al. CCR4‑NOT transcription complex subunit 2 regulates TRAIL sensitivity in non‑small‑cell lung cancer cells via the STAT3 pathway. (Int J Mol Med. 2020) {https://pubmed.ncbi.nlm.nih.gov/31894259/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 300 mM NaCl, 0.1mM DTT, 10% glycerol

Apoptosis

NP_003801

P50591

Human

MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG ELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG