Recombinant humaan TRAM/TICAM2-eiwit

Toll TIR domain-containing adapter molecule 2, also known as TICAM2, is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TICAM-2, a cytoplasmic protein, physically bridges TLR4 and TICAM-1 and functionally transmits LPS-TLR4 signaling to TICAM-1, which in turn activates IRF-3. It is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Recombinant human TICAM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TIR domain-containing adapter molecule 2, TICAM-2, TIRAP3, TIRP, TRAM

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.4 kDa (259aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

TICAM2, TIR domain-containing adapter molecule 2, TICAM-2, TIRAP3, TIRP, TRAM, ATGP1402-10 µg, ATGP1402-20 µg, ATGP1402-50 µg, ATGP1402-100 µg, ATGP1402-250 µg, ATGP1402-500 µg, ATGP1402-1 mg, ATGP1402-10, ATGP1402-20, ATGP1402-50, ATGP1402-100, ATGP1402-250, ATGP1402-500, ATGP1402-1

Yamamoto M., et al. (2002) J Immunol. 169:6668-6672.; ; Oshiumi H., et al. (2003) J Biol Chem. 278:49751-49762.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Immunology

NP_067681.1

Q86XR7

Human

MGIGKS KINSCPLSLS WGKRHSVDTS PGYHESDSKK SEDLSLCNVA EHSNTTEGPT GKQEGAQSVE EMFEEEAEEE VFLKFVILHA EDDTDEALRV QNLLQDDFGI KPGIIFAEMP CGRQHLQNLD DAVNGSAWTI LLLTENFLRD TWCNFQFYTS LMNSVNRQHK YNSVIPMRPL NNPLPRERTP FALQTINALE EESRGFPTQV ERIFQESVYK TQQTIWKETR NMVQRQFIA