Recombinant humaan TRANCE/RANK L/TNFSF11-eiwit

TNFSF11, also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TNFSF11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant human TNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

22.3 kDa (199aa) confirmed by MALDI-TOF

TRANCE , TNFSF11, RANK L, Tumor necrosis factor ligand superfamily member 11, OPGL, RANKL, ATGP1093-10 µg, ATGP1093-20 µg, ATGP1093-50 µg, ATGP1093-100 µg, ATGP1093-250 µg, ATGP1093-500 µg, ATGP1093-1 mg, ATGP1093-10, ATGP1093-20, ATGP1093-50, ATGP1093-100, ATGP1093-250, ATGP1093-500, ATGP1093-1

Lam J., et al. (2001) J. Clin. Invest. 108:971-979; ; Ito S., et al. (2002) J. Biol. Chem. 277:6631-6636

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Immunology

NP_003692

O14788

Human

MGSSHHHHHH SSGLVPRGSH MIRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID